Coding

Part:BBa_K3559000

Designed by: Scott Stacey   Group: iGEM20_Imperial_College   (2020-10-20)


BioBrick_BASIC_DAHP Synthase (Aro4) (K229L)

Biology

This is the CDS for the yeast gene ARO4 which codes for the aromatic amino acid biosynthesis enzyme 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (E.C.2.5.1.54). An amino acid substitution K229L has been made which has been shown in the literature to lead to higher activity through deleting negative regulation. This in turn leads to higher rates of aromatic amino acid biosynthesis.

Usage

This part is immediately surrounded by BASIC integrated prefix and suffix (iP/iS), allowing the part to be used in BASIC assembly. Surrounding the BASIC iP/iS are the BioBrick prefix and suffix. This means that the part allows for cloning with both the BASIC assembly method and with BioBrick assembly. This part would naturally suit any projects looking to optimise or increase aromatic amino acid biosynthesis in Saccharomyces cerevisiae.

Characterisation

As a result of the Covid pandemic, we were unable to test this part in the lab, and so we can not currently confirm it functions as intended.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8
    Illegal BsaI.rc site found at 1153


[edit]
Categories
//cds
//cds/biosynthesis
//chassis/eukaryote/yeast
Parameters
None